2QG3

CRYSTAL STRUCTURE OF A TYW3 METHYLTRANSFERASE-LIKE PROTEIN (AF_2059) FROM ARCHAEOGLOBUS FULGIDUS DSM 4304 AT 1.95 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979105
Spacegroup name	P 42
Unit cell lengths	59.334, 59.334, 109.102
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.628 - 1.950
R-factor	0.201
Rwork	0.199
R-free	0.25300
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.495
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.628	28.628	2.060
High resolution limit [Å]	1.950	6.170	1.950
Rmerge	0.088	0.022	0.756
Total number of observations		3247	14992
Number of reflections	27454
<I/σ(I)>	7.5	21.7	1
Completeness [%]	99.8	98.8	99.8
Redundancy	3.7	3.6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	NANODROP, 0.09M Citric acid, 0.01M Trisodium citrate, 20% MPD, Additive: 0.01 M Phenol, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 4.0
2	VAPOR DIFFUSION, SITTING DROP	4.1	293	NANODROP, 0.08M Citric acid, 0.02M Trisodium citrate, 22% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 4.1