2QG1
Crystal structure of the 11th PDZ domain of MPDZ (MUPP1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9182 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.409, 36.919, 63.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.800 - 1.400 |
| R-factor | 0.17252 |
| Rwork | 0.169 |
| R-free | 0.23418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2HE2 1tp3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.489 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.800 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.073 | 0.394 |
| Number of reflections | 15501 | |
| <I/σ(I)> | 13.5 | 2.6 |
| Completeness [%] | 96.6 | 94.7 |
| Redundancy | 4.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 0.8M (NH4)2SO4, 0.1M Citrate pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
