2QAG
Crystal structure of human septin trimer 2/6/7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07188 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 252.515, 252.515, 156.485 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.150 - 4.000 |
| R-factor | 0.37637 |
| Rwork | 0.376 |
| R-free | 0.39208 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.057 |
| RMSD bond angle | 4.719 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.379 | |
| Number of reflections | 42485 | |
| <I/σ(I)> | 11.42 | 3.98 |
| Completeness [%] | 98.9 | |
| Redundancy | 16.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.4M Ammonium Sulfate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
