2QA7
Crystal structure of Huntingtin-interacting protein 1 (HIP1) coiled-coil domain with a basic surface suitable for HIP-protein interactor (HIPPI)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-07 |
Detector | NOIR-1 |
Wavelength(s) | 0.96407, 0.97907, 0.97892 |
Spacegroup name | P 43 |
Unit cell lengths | 72.900, 72.900, 106.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.32 |
Rwork | 0.265 |
R-free | 0.32400 |
Structure solution method | MAD |
Starting model (for MR) | none |
RMSD bond length | 0.009 |
RMSD bond angle | 1.190 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.7LDz) |
Phasing software | RESOLVE (2.03) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.390 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.093 | 0.488 |
Total number of observations | 10273 | |
Number of reflections | 15320 | |
<I/σ(I)> | 10.3 | 2.9 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 7.07 | 6.72 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 15% PEG 3350, 0.1 M succinate, 0.2 M potassium sodium tartrate, 0.01 M nickle chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |