2Q9P

Human diphosphoinositol polyphosphate phosphohydrolase 1, Mg-F complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-19
Detector	Bruker Platinum 135
Spacegroup name	P 21 21 21
Unit cell lengths	45.193, 59.444, 62.547
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.210 - 1.650
R-factor	0.194
Rwork	0.192
R-free	0.23000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2fvv
RMSD bond length	0.020
RMSD bond angle	1.846
Data reduction software	SAINT
Data scaling software	SADABS
Phasing software	REFMAC (5.2.0019)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.750
High resolution limit [Å]	1.650	1.650
Number of reflections	20848
<I/σ(I)>	11.7	2.1
Completeness [%]	99.8	99.3
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	30% PEG 8000, 200 mM LiCl, 5 mM MgCl2, 20 mM NaF, 5 mM IP6, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K