2Q99
Crystal Structure of Saccharopine Dehydrogenase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9200,0.9794,0.9796,1.0080 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.680, 74.920, 74.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.970 - 1.640 |
Rwork | 0.187 |
R-free | 0.21600 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.08) |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.970 | 1.700 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.073 | 0.699 |
Number of reflections | 44946 | |
<I/σ(I)> | 11.3 | |
Completeness [%] | 98.8 | 89.3 |
Redundancy | 6.7 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% PEG 8000, 0.2 M calcium acetate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% PEG 8000, 0.2 M calcium acetate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% PEG 8000, 0.2 M calcium acetate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% PEG 8000, 0.2 M calcium acetate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |