2Q94

E. coli methionine aminopeptidase Mn-form with inhibitor A04

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	38.199, 60.605, 50.624
Unit cell angles	90.00, 104.80, 90.00

Refinement procedure

Resolution	19.910 - 1.630
Rwork	0.213
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xnz
RMSD bond length	0.005
RMSD bond angle	1.322
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.913	19.910	1.720
High resolution limit [Å]	1.630	5.150	1.630
Rmerge	0.044	0.034	0.363
Total number of observations		3165	13929
Number of reflections	27719
<I/σ(I)>	12.1	15.3	2
Completeness [%]	99.3	98.2	98
Redundancy	3.6	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	10-15% PEG 20000, 0.1 M MES (pH 6.5), vapor diffusion, hanging drop, temperature 291K