2Q8M

T-like Fructose-1,6-bisphosphatase from Escherichia coli with AMP, Glucose 6-phosphate, and Fructose 1,6-bisphosphate bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-29
Detector	NOIR-1
Wavelength(s)	0.979
Spacegroup name	P 41 2 2
Unit cell lengths	124.640, 124.640, 132.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.770 - 2.050
R-factor	0.212
Rwork	0.209
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gq1
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.410	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.068	0.255
Total number of observations		15355
Number of reflections	63542
<I/σ(I)>	9.2	3.5
Completeness [%]	96.6	95.7
Redundancy	2.43	2.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	Protein solution: (15 mg/mL enzyme (pH 7.4), 20 mM dithiothreitol (DTT), 0.1 mM EDTA, 5 mM Fru-1,6-P2, 5 mM MgCl2, and 5 mM AMP) Precipitant solution: (50 mM MES-NaOH, pH 6.5, 13% (w/v) PEG 10,000, and 20% (w/v) sucrose), pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K