2Q86
Structure of the mouse invariant NKT cell receptor Valpha14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.293, 150.518, 65.814 |
| Unit cell angles | 90.00, 117.04, 90.00 |
Refinement procedure
| Resolution | 37.350 - 1.850 |
| R-factor | 0.18009 |
| Rwork | 0.179 |
| R-free | 0.22262 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tcr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.519 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.040 | 0.327 |
| Number of reflections | 90127 | |
| <I/σ(I)> | 21.6 | 2 |
| Completeness [%] | 93.0 | 82.5 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 16% PEG 4000, 0.2 M magnesium acetate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
