2Q82

Crystal structure of core protein P7 from Pseudomonas phage phi12. Northeast Structural Genomics Target OC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-08
Detector	MAR CCD 165 mm
Wavelength(s)	0.97947
Spacegroup name	P 32 2 1
Unit cell lengths	75.499, 75.499, 46.486
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.830
R-factor	0.21
Rwork	0.210
R-free	0.22600
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.050	0.031	0.674
Number of reflections	29155
<I/σ(I)>	12
Completeness [%]	89.4	98.4	48.6
Redundancy	10.5	11.6	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	23% PEG10000, 100mM Sodium acetate, 10% n-Hexyl-D-glucoside, VAPOR DIFFUSION, HANGING DROP, temperature 293K