2Q81

Crystal Structure of the Miz-1 BTB/POZ domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	65.535, 128.522, 116.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.100
R-factor	0.184
Rwork	0.181
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nn2
RMSD bond length	0.011
RMSD bond angle	1.179
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MrBUMP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.260	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.052	0.300
Number of reflections	28911
<I/σ(I)>	21.7	6.1
Completeness [%]	99.8	100
Redundancy	6.1	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	20% PEG 3350, 20% PEG 400, 200 mM MgCl2, 100 mM HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K