2Q76
Mouse anti-hen egg white lysozyme antibody F10.6.6 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.431 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.760, 80.250, 91.500 |
| Unit cell angles | 90.00, 91.50, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Fab fragment of PDB 1P2C |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.195 |
| Number of reflections | 50300 | |
| <I/σ(I)> | 9.8 | 3.3 |
| Completeness [%] | 96.0 | 92 |
| Redundancy | 8.1 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 298 | The F10.6.6 Fab fragment was crystallized in a mother liquor containing 24% (w/v) PEG 1000, 0.2 M CaCl2 and 0.1 M acetate buffer pH 4.7. Large prisms of about 0.30 mm x 0.05 mm x 0.05 mm were obtained after several weeks. VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 4.70 |
