2Q5I
Crystal structure of apo S581L Glycyl-tRNA synthetase mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97650 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 92.530, 92.530, 246.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.270 - 2.800 |
| R-factor | 0.2144 |
| Rwork | 0.213 |
| R-free | 0.24919 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ati |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.987 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.108 | 0.734 |
| Number of reflections | 27432 | |
| <I/σ(I)> | 20.7 | 1.5 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 14.2 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG 3350, 0.2M Na2SO4, 0.1M Bis-Tris propane, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
