2Q5E
Crystal structure of human carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.643, 117.637, 170.134 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.510 |
| R-factor | 0.219 |
| Rwork | 0.216 |
| R-free | 0.27800 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.084 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.760 | 2.650 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.072 | 0.447 |
| Number of reflections | 72419 | |
| <I/σ(I)> | 18.6 | 2.6 |
| Completeness [%] | 96.5 | 81.7 |
| Redundancy | 5.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 2.8 M Sodium acetate, pH 7.0, VAPOR DIFFUSION, temperature 294K |
