2Q31
Actin Dimer Cross-linked Between Residues 41 and 374 and proteolytically cleaved by subtilisin between residues 47 and 48.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.253, 71.984, 54.703 |
| Unit cell angles | 90.00, 104.68, 90.00 |
Refinement procedure
| Resolution | 43.070 - 2.700 |
| R-factor | 0.251 |
| Rwork | 0.249 |
| R-free | 0.28800 |
| Structure solution method | difference Fourier |
| Starting model (for MR) | 2q1n |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.035 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.820 | 2.700 |
| Rmerge | 0.176 | 0.091 | 0.418 |
| Number of reflections | 19970 | ||
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 87.5 | 99.4 | 55.8 |
| Redundancy | 2.8 | 3.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 30% MPD, 0.1 M sodium acetate, 0.01M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
