2Q2C
Crystal structures of the arginine-, lysine-, histidine-binding protein ArtJ from the thermophilic bacterium Geobacillus stearothermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.95097 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.070, 102.200, 70.535 |
Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.350 |
R-factor | 0.27 |
Rwork | 0.268 |
R-free | 0.33300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.878 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.139 | 0.364 |
Number of reflections | 36922 | |
<I/σ(I)> | 6.4 | 2.2 |
Completeness [%] | 91.6 | 80.1 |
Redundancy | 2.9 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M ammoniumsulfate, 30.5 % w/v polyethylene glycol (PEG) 2000 , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |