2Q22

Crystal structure of uncharacterized protein (YP_323524.1) from Anabaena variabilis ATCC 29413 at 2.11 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97939
Spacegroup name	P 32
Unit cell lengths	103.374, 103.374, 42.086
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.841 - 2.110
R-factor	0.193
Rwork	0.191
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.521
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.841	29.840	2.160
High resolution limit [Å]	2.110	9.440	2.110
Rmerge	0.118	0.051	0.651
Total number of observations		1158	7880
Number of reflections	28912
<I/σ(I)>	5.2	11.4	1.2
Completeness [%]	100.0	96.4	100
Redundancy	3.8	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.18	293	NANODROP, 5.0% PEG 1000, 48.3% Ethylene glycol, 0.1M Sodium acetate pH 4.18, VAPOR DIFFUSION, SITTING DROP, temperature 293K