2PZZ
2.2 A resolution crystal structure of UPF0201 protein from Methanococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.520, 50.150, 73.820 |
| Unit cell angles | 70.34, 72.61, 84.30 |
Refinement procedure
| Resolution | 47.230 - 2.200 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.29900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.278 |
| Number of reflections | 30128 | |
| <I/σ(I)> | 14.6 | 2.4 |
| Completeness [%] | 98.9 | 90.9 |
| Redundancy | 7.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Ammonium acetate, HEPES Buffer, PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
