2PZX
Structure of the methuselah ectodomain with peptide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-04 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 42 |
| Unit cell lengths | 94.233, 94.233, 173.833 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.990 - 3.500 |
| R-factor | 0.376 |
| Rwork | 0.376 |
| R-free | 0.40700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fjr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.155 | 0.370 |
| Number of reflections | 18125 | |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 95.3 | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 1.7 M ammonium sulfate 2% w/v PEG 400 0.1 M HEPES-KOH pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.50 |
