2PZS
Phi29 DNA polymerase complexed with primer-template DNA (post-translocation binary complex)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.10000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 216.633, 146.292, 115.054 |
Unit cell angles | 90.00, 117.93, 90.00 |
Refinement procedure
Resolution | 45.220 - 2.600 |
R-factor | 0.22452 |
Rwork | 0.219 |
R-free | 0.27524 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | copy c of 1XHX without residues 358-394 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.239 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.152 | 0.773 |
Number of reflections | 95762 | |
<I/σ(I)> | 5.8 | 1 |
Completeness [%] | 98.4 | 95.1 |
Redundancy | 2.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris-HCl, 20% PEG 10000, 200 mM magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | sodium acetate | ||
2 | 1 | 1 | ammonium acetate | ||
3 | 1 | 1 | PEG 4000 | ||
4 | 1 | 2 | sodium acetate | ||
5 | 1 | 2 | ammonium acetate | ||
6 | 1 | 2 | PEG 4000 |