2PYR
PHOTOACTIVE YELLOW PROTEIN, 1 NANOSECOND INTERMEDIATE (287K)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID09 |
| Synchrotron site | ESRF |
| Beamline | ID09 |
| Temperature [K] | 287 |
| Detector technology | CCD AREA DETECTOR |
| Collection date | 1996-11 |
| Detector | THOMPSON |
| Spacegroup name | P 63 |
| Unit cell lengths | 66.900, 66.900, 40.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.18 * |
| R-free | 0.18500 * |
| Data reduction software | LaueView |
| Data scaling software | LaueView |
| Phasing software | X-PLOR |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.570 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.092 | |
| Number of reflections | 13027 | |
| Completeness [%] | 83.4 | 44.8 |
| Redundancy | 14 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | seeding * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 2.5 (M) | |
| 3 | 1 | drop | sodium phosphate | 20 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.5 (M) |
