2PYL
Phi29 DNA polymerase complexed with primer-template DNA and incoming nucleotide substrates (ternary complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.922, 78.199, 157.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.420 - 2.200 |
| R-factor | 0.19732 |
| Rwork | 0.191 |
| R-free | 0.25291 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | copy c of 1XHX without residues 359-394 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.271 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.166 | 0.850 |
| Number of reflections | 36737 | |
| <I/σ(I)> | 8.9 | 1.2 |
| Completeness [%] | 97.0 | 83.7 |
| Redundancy | 4.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 100 mM CHES, 15-20% PEG 8000, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CHES | ||
| 2 | 1 | 1 | PEG 8000 | ||
| 3 | 1 | 2 | CHES | ||
| 4 | 1 | 2 | PEG 8000 |
