2PY8

RbcX

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97947,0.97969,0.97182
Spacegroup name	P 43 21 2
Unit cell lengths	129.832, 129.832, 92.742
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.500 - 2.450
R-factor	0.211
Rwork	0.209
R-free	0.24900
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.137
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.059	0.541
Number of reflections	29666
<I/σ(I)>	16.7
Completeness [%]	99.9	100
Redundancy	7.7	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	30% PEG 400, 0.1M potassium/sodium phosphate, 0.2M lithium sulfate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	30% PEG 400, 0.1M potassium/sodium phosphate, 0.2M lithium sulfate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	30% PEG 400, 0.1M potassium/sodium phosphate, 0.2M lithium sulfate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K