2PXS

Crystal Structure of N66D Mutant of Green Fluorescent Protein from Zoanthus sp. at 2.2 A Resolution (Mature State)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 62 2 2
Unit cell lengths	101.510, 101.510, 271.217
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.300 - 2.200
R-factor	0.178
Rwork	0.176
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	zGFP506
RMSD bond length	0.018
RMSD bond angle	1.793
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.066	0.048	0.663
Number of reflections	42936
<I/σ(I)>	11.3
Completeness [%]	99.8	98.8	100
Redundancy	14.4	13.3	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M bis-tris propane, 1.8 M tri-ammonium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K