2PX3

Crystal structure of FlhF complexed with GTP/Mg(2+)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	80.965, 80.965, 126.137
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.760 - 3.200
Rwork	0.250
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	G domain of FtsY from Sulfolobus solfataricus
RMSD bond length	0.008
RMSD bond angle	1.900
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.200	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	7364
<I/σ(I)>	19.4	4.2
Completeness [%]	99.9	99.9
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	10-13 % (w/v) PEG6000, 4% (v/v) 2-methyl-2,4-pentanediol, 0.1 M MOPS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K