2PWV
tRNA guanine transglycosylase E235Q mutant in complex with preQ0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.757, 65.175, 70.455 |
| Unit cell angles | 90.00, 96.12, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.2006 |
| Rwork | 0.201 |
| R-free | 0.24510 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | 1p0b |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.026 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.059 | 0.491 |
| Number of reflections | 40368 | |
| <I/σ(I)> | 12 | 2.2 |
| Completeness [%] | 1.0 | 0.969 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
