2PWU

tRNA guanine transglycosylase in complex with guanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2007-01-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	C 1 2 1
Unit cell lengths	90.151, 64.503, 70.769
Unit cell angles	90.00, 92.79, 90.00

Refinement procedure

Resolution	10.000 - 1.770
Rwork	0.200
Structure solution method	AB INITIO
Starting model (for MR)	1p0b
RMSD bond length	0.006
RMSD bond angle	0.023
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.035	0.389
Number of reflections	34387
<I/σ(I)>	22	2.3
Completeness [%]	94.8	0.839
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K