2PWJ
Structure of a mitochondrial type II peroxiredoxin from Pisum sativum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-02 |
| Detector | BRUKER PLATINUM 200 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.880, 66.400, 77.230 |
| Unit cell angles | 102.94, 104.44, 99.07 |
Refinement procedure
| Resolution | 14.980 - 2.800 |
| R-factor | 0.298 |
| Rwork | 0.277 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | model generated by EasyPed3D Web Server 1.0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.900 |
| Data reduction software | SAINT |
| Data scaling software | SAINT (V7.12A) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 71.900 | 2.480 | |
| High resolution limit [Å] | 2.390 | 5.170 | 2.400 |
| Number of reflections | 30073 | ||
| <I/σ(I)> | 15.416 | ||
| Completeness [%] | 65.8 | ||
| Redundancy | 3.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG2000, citrate, NaCl, DTT, 2-propanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
