2PWH
Crystal structure of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9803 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.580, 71.540, 81.750 |
| Unit cell angles | 67.66, 73.04, 70.44 |
Refinement procedure
| Resolution | 19.560 - 2.000 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.21500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1zja |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 80624 | |
| <I/σ(I)> | 8.9 | 2.84 |
| Completeness [%] | 96.6 | |
| Redundancy | 2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 12% PEG20000, 0.1M Na cacodylate, 0.01M L-cysteine, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
