2PVV
Structure of human glutamate carboxypeptidase II (GCPII) in complex with L-serine-O-sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-23 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 101.617, 130.220, 158.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.110 |
| R-factor | 0.17622 |
| Rwork | 0.174 |
| R-free | 0.21214 |
| Structure solution method | Difference Fourier |
| Starting model (for MR) | 2oot |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.803 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.097 | 0.505 |
| Number of reflections | 60304 | |
| <I/σ(I)> | 15.4 | 3.7 |
| Completeness [%] | 98.9 | 98.4 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 33% (v/v) pentaerythritol propoxylate PO/OH 5/4, 1 3% (w/v) PEG 3350, 100 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
