2PVO
Crystal srtucture of the ternary complex between thioredoxin f, ferredoxin, and ferredoxin: thioredoxin reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97970 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 130.426, 130.426, 63.837 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 3.400 |
Rwork | 0.202 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 7.100 | 3.300 |
Rmerge | 0.195 | 0.069 | 0.675 |
Number of reflections | 9413 | ||
<I/σ(I)> | 4.5 | ||
Completeness [%] | 97.0 | 89.1 | 99.5 |
Redundancy | 8.2 | 7.2 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |