2PV0
DNA methyltransferase 3 like protein (DNMT3L)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2006-10-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0,1.2199,1.2815,1.2826,1.2830 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 267.200, 267.200, 149.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.740 - 3.300 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.27200 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.410 |
High resolution limit [Å] | 3.290 | 3.290 |
Rmerge | 0.095 | 0.489 |
Number of reflections | 47346 | |
<I/σ(I)> | 13.4 | 3.9 |
Completeness [%] | 98.9 | 99.1 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 300 | 0.4-0.6 M sodium citrate, 0.1 M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 300 | 0.4-0.6 M sodium citrate, 0.1 M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |