2PUY
Crystal Structure of the BHC80 PHD finger
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2006-02-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97917, 1.28295, 1.28341 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.511, 25.386, 62.282 |
| Unit cell angles | 90.00, 96.93, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.430 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.23100 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.470 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.089 | 0.237 |
| Number of reflections | 23287 | |
| <I/σ(I)> | 16 | 8.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.4 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20% Isopropanol, 5-10% polyethylene glycol 4000, 100mM MES 6.2-6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
