2PTM
Structure and rearrangements in the carboxy-terminal region of SpIH channels
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Detector technology | CCD |
Collection date | 2004-04-14 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 92.354, 92.354, 63.646 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.205 - 1.930 |
Rwork | 0.187 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q5o |
RMSD bond length | 0.004 |
RMSD bond angle | 0.633 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.930 | 1.930 |
Number of reflections | 21201 | |
<I/σ(I)> | 30.5 | 7.9 |
Completeness [%] | 99.6 | 100 |
Redundancy | 13.1 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 10 % w/v PEG 6000, 0.5 M NaCl, 20 % glycerol, 0.1 M HEPES, 10 mM hexamine cobalt trichloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |