2PT7
Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97590 |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.851, 86.900, 104.129 |
| Unit cell angles | 111.04, 95.98, 104.94 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.23185 |
| Rwork | 0.230 |
| R-free | 0.26966 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g6o |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.197 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.916 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.091 | 0.243 |
| Number of reflections | 100842 | |
| <I/σ(I)> | 5 | 2.9 |
| Completeness [%] | 96.3 | 96.5 |
| Redundancy | 3.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 100mM Mes, 8% polyethylene glycol 8,000, 10mM ethylene-diamine-tetra-acetic acid, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
