2PST

1.8A Crystal Structure of the PA2412 protein from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	113
Detector technology	CCD
Collection date	2005-07-30
Detector	ADSC QUANTUM 4
Spacegroup name	P 41
Unit cell lengths	51.543, 51.543, 32.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.500 - 1.800
R-factor	0.194
Rwork	0.192
R-free	0.23200
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.265
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.500	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.072	0.285
Number of reflections	7822
<I/σ(I)>	13.9	2.3
Completeness [%]	96.3	72.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5	277	Microbatch 1:1 with 33-37% polyethylene propoxylate 426, 50 mM Succinate, pH 5.0, temperature 277K
1	MICROBATCH	5	277	Microbatch 1:1 with 33-37% polyethylene propoxylate 426, 50 mM Succinate, pH 5.0, temperature 277K
1	MICROBATCH	5	277	Microbatch 1:1 with 33-37% polyethylene propoxylate 426, 50 mM Succinate, pH 5.0, temperature 277K
1	MICROBATCH	5	277	Microbatch 1:1 with 33-37% polyethylene propoxylate 426, 50 mM Succinate, pH 5.0, temperature 277K