2PSR

HUMAN PSORIASIN (S100A7) CA2+ AND ZN2+ BOUND FORM (CRYSTAL FORM II)

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-09
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	51.958, 51.958, 116.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.050
R-factor	0.27 *
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1psr
RMSD bond length	0.005
RMSD bond angle	0.019
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.100	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.113 *	0.423 *
Number of reflections	10791
<I/σ(I)>	15.9	5
Completeness [%]	98.9	100
Redundancy	7	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.7	4 *	or 20 degrees centigrade *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	14 (mg/ml)
2	1	1	PEG4000	5-12 (%(v/v))
3	1	1	glycerol	10 (%(v/v))
4	1	1		20 (mM)