2PSP
Porcine pancreatic spasmolytic polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 60.600, 60.600, 113.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.25800 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 26.760 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.950 |
| Rmerge | 0.058 |
| Number of reflections | 15652 |
| Completeness [%] | 87.3 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | 2.0 (M) | ||
| 3 | 1 | reservoir | PEG400 | 7 (%(w/v)) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) |
