2PSN
Crystal structure of enolase1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1. |
| Spacegroup name | P 42 |
| Unit cell lengths | 192.796, 192.796, 65.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.087 | 0.239 |
| Number of reflections | 121573 | |
| <I/σ(I)> | 12.1 | 6.1 |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 6.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 20% to 24% (w/v) PEG 3350, 100 mM Tris-HCl (pH 7.5), 200 mM ammoniumsulfate, 1 mM DTT., VAPOR DIFFUSION, HANGING DROP, temperature 291K |
