2PSM

Crystal structure of Interleukin 15 in complex with Interleukin 15 receptor alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9793
Spacegroup name	P 4 21 2
Unit cell lengths	118.450, 118.450, 76.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.190
Rwork	0.224
R-free	0.23600
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.180
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.270
High resolution limit [Å]	2.190	2.190
Number of reflections	53064
<I/σ(I)>	23	18.6
Completeness [%]	99.9	100
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	1.7M Na/K Phosphate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K