2PSF
Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Collection date | 2005-09-17 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.178, 82.279, 90.379 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.400 |
R-factor | 0.17366 |
Rwork | 0.173 |
R-free | 0.19406 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.209 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.400 |
Number of reflections | 111557 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.4M sodium acetate, 20% w/v PEG 3350, 1.8mM n-Decyl-beta-D-thiomaltoside, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |