2PR8
crystal structure of aminoglycoside N-acetyltransferase AAC(6')-Ib11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2005-09-26 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9340 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.620, 85.370, 150.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.100 - 2.100 |
R-factor | 0.201 |
Rwork | 0.196 |
R-free | 0.25200 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.687 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.100 | 2.230 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.045 | 0.190 |
Number of reflections | 26857 | |
<I/σ(I)> | 20.42 | 7.3 |
Completeness [%] | 98.2 | 97.3 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0,1 M Hepes pH7.5 1.5 M lithium sulfate 3 % isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0,1 M Hepes pH7.5 1.5 M lithium sulfate 3 % isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0,1 M Hepes pH7.5 1.5 M lithium sulfate 3 % isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0,1 M Hepes pH7.5 1.5 M lithium sulfate 3 % isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |