2PQ0

Crystal structure of Hyopthetical protein (gk_1056) from geobacillus Kaustophilus HTA426

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-02
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.97882, 0.97938, 0.90000
Spacegroup name	P 41 21 2
Unit cell lengths	114.050, 114.050, 182.309
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.070 - 2.600
R-factor	0.208
Rwork	0.208
R-free	0.24100
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.066	0.356
Number of reflections	67421
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	95mM NaCl, 95mM Hepes, 1.52M Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K