2PO4

X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-30
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1, 0.9790, 0.9793, 0.9649
Spacegroup name	P 21 21 21
Unit cell lengths	83.047, 103.258, 159.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
Rwork	0.219
R-free	0.24800
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.071	0.596
Number of reflections	91553
<I/σ(I)>	10.1
Completeness [%]	98.3	98.9
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	300	10mM Tris-HCl (pH 8), 100mM NaCl and 0.1mM EDTA , pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	5	300	10mM Tris-HCl (pH 8), 100mM NaCl and 0.1mM EDTA , pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	5	300	10mM Tris-HCl (pH 8), 100mM NaCl and 0.1mM EDTA , pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 300K