2PO1
Crystal structure of the P. abyssi exosome RNase PH ring complexed with a single stranded 10-mer poly(A) RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.427 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 93.555, 93.555, 125.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.940 |
| R-factor | 0.19016 |
| Rwork | 0.187 |
| R-free | 0.24243 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pnz |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.065 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.780 | 2.060 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 46981 | |
| <I/σ(I)> | 14.9 | 3.5 |
| Completeness [%] | 98.2 | 90.1 |
| Redundancy | 11 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1 M Bis-Tris, 45% MPD and 0.1 M LiCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Bis-Tris | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | LiC | ||
| 4 | 1 | 2 | Bis-Tris | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | LiC |
