2PN4
Crystal Structure of Hepatitis C Virus IRES Subdomain IIa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-03-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.286, 48.730, 119.999 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.980 - 2.320 |
| R-factor | 0.26405 |
| Rwork | 0.261 |
| R-free | 0.32037 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pn3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.835 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.980 | 2.430 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.108 | 0.714 |
| Number of reflections | 12228 | |
| <I/σ(I)> | 17.5 | 2.4 |
| Completeness [%] | 95.9 | 100 |
| Redundancy | 5.5 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.2 | 290 | 30% MPD, 80mM SrCl2, 25mM NaCl, 50mM Cacodylate equilibrated with 60%MPD, pH 6.2, EVAPORATION, temperature 290.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 30% MPD | ||
| 2 | 1 | 1 | SrCl2 | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | Cacodylate | ||
| 5 | 1 | 2 | 60%MPD |
