2PN3
Crystal Structure of Hepatitis C Virus IRES Subdomain IIa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2004-10-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9203, 0.9197, 0.90686 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 48.945, 48.945, 120.942 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.900 |
| R-factor | 0.22969 |
| Rwork | 0.229 |
| R-free | 0.28263 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.885 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 | 0.345 |
| Number of reflections | 3420 | |
| <I/σ(I)> | 27.5 | 4.6 |
| Completeness [%] | 93.7 | 75.1 |
| Redundancy | 11.2 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.2 | 290 | 5% MPD, 5mM MgSO4, 12.5mM NaCl, 50mM KCl, 25mM Cacodylate equilibrated with 65%MPD, pH 6.2, EVAPORATION, temperature 290.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 5% MPD | ||
| 2 | 1 | 1 | MgSO4 | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | KCl | ||
| 5 | 1 | 1 | Cacodylate | ||
| 6 | 1 | 2 | 65% MPD |
