2PMY

EF-hand domain of human RASEF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-01-17
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	61.259, 61.259, 101.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
Rwork	0.229
R-free	0.26340
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.235
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.11)
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.137	0.053
Number of reflections	18809	1887	1889
<I/σ(I)>	8
Completeness [%]	100.0
Redundancy	14.9	14.4	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	291	0.6M Ammonium sulfate, 0.1M Sodium cacodylate, 0.01M Magnesium chloride, pH 5.0, VAPOR DIFFUSION, temperature 291K