2PMI
Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway with Bound ATP-gamma-S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-03-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 146.592, 146.592, 212.848 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.980 - 2.900 |
| R-factor | 0.289 |
| Rwork | 0.289 |
| R-free | 0.32500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pk9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.980 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.099 | 0.248 |
| Number of reflections | 53704 | |
| <I/σ(I)> | 11 | 2.4 |
| Completeness [%] | 91.1 | 75.6 |
| Redundancy | 3.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 9ul 12% PEG 10000, 10% glycerol, 0.05 M strontium chloride (SrCl2), 10 mM Tris (2-carboxyethyl) phosphine (TCEP, 0.1 M 2-morpholinoethanesulfonic acid (MES), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
