2PMB
Crystal structure of predicted nucleotide-binding protein from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-30 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.802, 178.222, 89.513 |
| Unit cell angles | 90.00, 103.78, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.990 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.26600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.181 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.131 | 0.610 |
| Number of reflections | 123599 | |
| <I/σ(I)> | 3.4 | 0.6 |
| Completeness [%] | 94.8 | 83.7 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 294 | 0.1M SODIUM-POTASSIUM PHOSPHATE pH 6.2, 20% PEG 1000, 0.2M SODIUM CHLORIDE, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 294K, pH 6.20 |
